A375
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Description
Description: Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1973;51:1417
Hyperlink to ECACC Cell Line Data Sheet: 88113005
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