Description: Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice. The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. The following references provide more information about this tumour status: Martínez-Ramírez et al. 2003. Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques. Cancer Genet Cytogenet. Mar;141(2):138-42. PMID: 12606131 Coleman and Roberts, 2004. Re: Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques. Cancer Genet Cytogenet. Jan 1;148(1):86. PMID: 14697648 Teicher et al., 2011. Characteristics of human Ewing/PNET sarcoma models. Ann Saudi Med. Mar-Apr; 31(2): 174-182. PMCID: PMC3102479.

Also Known As:

Species: Human

Tissue: muscle

Growth Properties: Adherent

Morphology: Epithelial-like

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: J Nat Cancer Inst 1973;51:1417. PMID: 4357758; Am J Pathol 1993;142:49. PMID: 8380955; Cancer Genet Cytogenet 2003 Mar;141(2):138-42. PMID: 12606131; Cancer Genet Cytogenet. 2004 Jan 1;148(1):86. PMID: 14697648; Ann Saudi Med. 2011 Mar-Apr; 31(2): 174?182. PMCID: PMC3102479

Hyperlink to ECACC Cell Line Data Sheet: 85111504