Description: AB1-HA is a mouse mesothelioma cell line transfected with influenza haemaglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting cell line, denoted AB-1 was transfected with influenza haemaglutinin gene (HA) in order to generate an endogenous tumour antigen.

Organism: Mouse (Mus musculus)

Strain: BALB/c

Tissue: Mesothelium

Growth Properties: Adherent

Morphology: Epithelial. The cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence.

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS) + 400μg/ml G418.

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 2 times per week.

Subcultivation Ratio: 1:10-1:20. Seeding density 0.7 x 10⁴cells/cm². Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. Flasks will require passaging twice weekly at this seeding density.

Culture Conditions: Incubate the culture at 37°C with 5% CO₂.

Cryoprotectant Medium: 10% DMSO + 90% FCS.

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: AB-1 HA cells are tumourigenic in syngeneic immunocompetent mice.

Depositor: Richard Lake – University of Western Australia

References:

For AB-1 HA:

Marzo AL, Lake RA, Robinson BW, Scott B.

T-cell receptor transgenic analysis of tumor-specific CD8 and CD4 responses in the eradication of solid tumors.

Cancer Res 1999 59(5):1071-9.

PubMed 10070965

For AB-1 line derivation:

Davis MR, Manning LS, Whitaker D, Garlepp MJ, Robinson BW (1992)

Establishment of a murine model of malignant mesothelioma. 

Int J Cancer 52: 881-886.

PubMed 1459729

Cellosaurus: CVCL_G361