Description: AE17 is a mouse mesothelioma cell line. Mice (C57 Black/6J strain female 6-8 weeks) were exposed to crocidiolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid.

Description Key Words: Mouse mesothelioma

Also Known As: No other names

Organism: Mouse (Mus musculus)

Strain: C57 Black/6J

Tissue: Mesothelium; malignant mesothelial cells were obtained from ascites fluid.

Growth Properties: Adherent

Morphology: Epithelial-like

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 5% Foetal Calf Serum (FCS)

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 2-3 times per week.

Subcultivation Ratio: 1:16 – 1:32. Seeding density 0.6 x10⁴ cells/cm². Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

Culture Conditions: Incubate the culture at 37°C with 5% CO₂.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: Cells are tumourigenic in syngeneic imunocompetent mice. Cells expressed Class-I, but not Class-II, H2 antigens. The percentage of Class-I positive cells ranged from 21-99% for H-2Dk and from 9-99% for H-2Kk.

Depositor: Richard Lake – University of Western Australia

References:

Connie Jackaman, Christine S. Bundell, Beverley F. Kinnear, Alison M. Smith, Pierre Filion, Deborah van Hagen, Bruce W.S. Robinson and Delilah J. Nelson.

IL-2 Intratumoral Immunotherapy Enhances CD8+ T Cells That Mediate Destruction of Tumor Cells and Tumor-Associated Vasculature: A novel Mechanism for IL-2. J Immunol 2003; 171:5051-5063.

PubMed: 14607902.

Cellosaurus: CVCL_4408