AC2
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Description
Description: Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.
Also Known As:
Species: Mouse
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37°C. Cells will rapidly die at high densities above 5×100,000 cells/ml. Culture very sensitive to agitation.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1984;81:1208; Lymphokines 1984;9:153
Hyperlink to ECACC Cell Line Data Sheet: 86061902
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