AFRC IAH-CC62
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Description
Description: The hybridoma IAH-CC62 secretes antibodies reactive with bovine CD26 – this was formerly known as WC10. This non-lineage specific antigen is present on bovine leukocytes and has a MW of 115 kDa and 39kDa. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentractions appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Also Known As:
Species: Mouse
Growth Medium: RPMI 1640 + 2 mM glutamine + HT supplement + 10% FBS
Subculturing Procedure: Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr. C.J. Howard, Institute for Animal Health, Compton, Newbury
Originator: Yes
References: Gliddon D.R. Howard C.J. European Journal of Immunology 32 pp. 1472-81
Hyperlink to ECACC Cell Line Data Sheet: 99011334
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