Description: A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

Also Known As:

Species: Human

Tissue: Brain

Growth Properties:

Morphology: Fusiform, can form heaps of cells

Growth Medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC.

Release Conditions: No

Depositor: Dr A Notarangelo, IRCCS-CASA, Sollievo Della Sofferenza Hospital, Viale Cappuccini 71013, San Giovanni Rotondo (Fg), Italy

Originator: Yes

References: Notarangelo A1, Trombetta D2, D’Angelo V3, Parrella P2, Palumbo O1, Storlazzi CT4, Impera L4, Muscarella LA2, La Torre A2, Affuso A5, Fazio VM2, Carella M1, Zelante L1.2014 Establishment and genetic characterization of ANGM-CSS, a novel, immortal cell line derived from a human glioblastoma multiforme. Int J Oncol. 44(3):717-24. PMID: 24366606.

Hyperlink to ECACC Cell Line Data Sheet: 08040401