AT-3.1
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Description
Description: AT-3.1 is a subclone of the AT-3 cell line being one of a series of related variants originating from a single spontaneous adenocarcinoma in the dorsal lobe of the prostate of an aged male inbred Copenhagen rat. AT-3.1 was derived from an anaplastic tumour with a high rate of metastases and is androgen-independent.
Also Known As:
Species: Rat
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr J Isaacs, Johns Hopkins Oncology Centre, Baltimore MD
Originator: Yes
References: Prostate 1988; 13:165
Hyperlink to ECACC Cell Line Data Sheet: 94101451
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