ATHOS
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Description
Description: The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (ECACC catalogue no. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.
Also Known As:
Species: Porcine
Tissue: thyroid
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Click:RPMI (1:1) + 10µg/ml Insulin + 10nM Hydrocortisone + 5µg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5×100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
Release Conditions: No
Depositor: Dr G Fayet/Dr S Hovsepian, Laboratoire de Biochimie Medicale, Faculte de Medecine, Marseille, F-13385
Originator: Yes
References: Cell Mol Biol 1993;39:427; Horm Metab Res 1993;25:238
Hyperlink to ECACC Cell Line Data Sheet: 93122325
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