B82
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Description
Description: Derivative of NCTC clone 929 (ECACC catalogue no. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.
Also Known As:
Species: Mouse
Tissue: adipose
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Exp Cell Res 1966;41:190; J Cell Sci 1969;5:93
Hyperlink to ECACC Cell Line Data Sheet: 85011408
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