BC3H1
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Description
Description: Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
Also Known As:
Species: Mouse
Tissue: brain
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Cell Biol 1989;108:1403
Hyperlink to ECACC Cell Line Data Sheet: 86093001
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