BHK TK
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Description
Description: BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30µg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.
Also Known As:
Species: Hamster
Tissue: kidney
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Nature 1964;203:1355; Nature 1966;211:250
Hyperlink to ECACC Cell Line Data Sheet: 85011423
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