Description: The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

Also Known As:

Species: Human

Tissue: Lymph node, auxillary

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 1-5 x 100,000 cells/ml;5% CO2; 37°C

Release Conditions: No

Depositor: Prof I. Palyi, National Institute of Oncology, Budapest, Hungary

Originator: Yes

References: Periodicum Biol. 1982;84:357, Tissue Culture and Reticuloendothelial system Res 1984;287, Haematologica 1995; 80: 206

Hyperlink to ECACC Cell Line Data Sheet: 98070109