C-4I
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Description
Description: Established from a cervical carcinoma of a 41 year old Caucasian female. Cells form stratified, epithelial colonies and retain properties of squamous cell differentiation in long term culture. They are morphologically and cytogenetically distinct from C-4 II (ECACC catalogue no. 88102701). Reported to contain human papilloma virus (Am. J. Pathol. 119:361-366). Handle as potentially biohazardous material.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: W aymouth’s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1962;28:605; J Nat Cancer Inst 1969;43:151; J Nat Cancer Inst 1969;43:175; J Nat Cancer Inst 1972;48:1589
Hyperlink to ECACC Cell Line Data Sheet: 89051702
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