C32TG (melanoma)
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Description
Description: This cell line has been derived by the selection of the human amelanotic melanoma cell line C32 for 6-thioguanine resistance. The parent cell line C32 was established from a melanoma from a 53 year old Caucasian male. The cells are resistant to 6-thioguanine and sensitive to HAT medium. They are deficient in hypoxanthine ? guanine phosphoribosyl transferase (HGPRT-). The cell line is also known as C32-r16TG. The cells are tumorigenic in nude mice inoculated subcutaneously with 10E7 cells.
Also Known As:
Species: Human
Tissue: Skin
Growth Properties: Adherent
Morphology:
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.050mM 6-thioguanine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80 %) 1:2 to 1:4 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO2.
Release Conditions: No
Depositor:
Originator: No
References: Chen TR, Cytogenet Cell Genet 1983, 35(3):181-189. Chen TR & Shaw MW, Cancer Res 1973, 33(9):2042-2047. Chen TR, J Natl Cancer Inst 1978, 61(2):277-284.
Hyperlink to ECACC Cell Line Data Sheet: 96020925
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