C3H/MCA clone 16
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Description
Description: Derived by exposing C3H/10T1/2 clone 8 cells with 3-methylcholanthrene for 24 hours. Subsequently they lost the property of contact inhibition. Inoculation of 100,000 cells or more into syngenic mice has been reported to produce fibrosarcoma.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: BME (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2×1,000 to 2×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1973;33:3231; Cancer Res 1973;33:3239 Nature 1976;260:710 Nature 1976;264:442 Virology 1975;65:392
Hyperlink to ECACC Cell Line Data Sheet: 90110523
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