C57/B1
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Description
Description: Spontaneous tumour of C57 black/6J mouse originally obtained from Dr Parrish of Australian National University, Canberra. Rendered HGPRT- by selection in 20µg/ml 6-thioguanine.
Also Known As:
Species: Mouse
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) at a maximum dilution of 1:10 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Monolayers will detach if monolayer becomes over-confluent.
Release Conditions: No
Depositor:
Originator: No
References: J Cell Sci 1977;42:217
Hyperlink to ECACC Cell Line Data Sheet: 85011438
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