Description: The Me-series of esotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. Reference cultures designated CARM-lines (CARM-L12 TG3 11120833 and CARM-L1 TG3 11120835) were derived from Me9-TG3 (ECACC Catalogue number 11120841) by cloning the cells three times in soft agar. Each line was selected on the basis of their epithelial or fibroblastic phenotype. The cell lines are metastatic in a syngeneic host.

Also Known As:

Species: Rat (PVG/c Norweigan Hooded)

Tissue: lung

Growth Properties: Adherent

Morphology: Epithelial and spindle shaped biphasic morphology

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².

Release Conditions: No

Depositor: DG Brown, MRC Toxicology Unit, Hodgkin Building, Leicester LE2 4UE

Originator: Yes

References: Brown DG, Wagner JC, Wagner MM 1980 Failure to demonstrate tumour-associated transplantation antigens on asbestos-induced mesotheliomas in rats. Br J Cancer. 42(5):797-801.PMID: 7459217

Hyperlink to ECACC Cell Line Data Sheet: 11120835