CCB
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Description
Description: The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.
Also Known As:
Species: Common Carp (Cyprinus carpio carpio)
Tissue: Brain
Growth Properties: Adherent
Morphology: Squamous
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). 10mM HEPES can be included if a CO2 incubator is not available.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25°C.
Release Conditions: No
Depositor:
Originator:
References: Neukirch M, Bottcher K, Bunnarjirakul S (1999) Isolation of a virus from koi with altered gills. Bull Eur Assoc Fish Pathol, 19:221-224.
Hyperlink to ECACC Cell Line Data Sheet: 10072802
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