Description: This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, EX-CELL® ACF CHO Medium (Sigma C5467).

Also Known As:

Species: Hamster

Tissue: Ovary

Growth Properties: Aggregates in suspension

Morphology: Suspension

Growth Medium: EX-CELL® ACF CHO medium (Sigma C5467) + 8mM Glutamine. When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.

Subculturing Procedure: Maintain cultures between approximately 3-9×100,000 cells/ml; 5% CO2; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 100,000 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days.

Release Conditions: No

Depositor: Dr. E. Penn, Culture Collections, Public Health England, Porton Down, Salisbury, SP4 0JG, UK

Originator: Yes

References: None Specified By Depositor

Hyperlink to ECACC Cell Line Data Sheet: 13080801