CHO-K1/SF
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Description
Description: This MEM adapted sub-line of CHO-KI is used in Pertussis Toxin research and for quality control of batch production.
Also Known As:
Species: Hamster
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Biol Stand 1985;13:61; Vaccine 1988;6:29; FEMS Microbiol Letters 1987;44:141
Hyperlink to ECACC Cell Line Data Sheet: 93061607
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