Clone M-3
Mouse (CxDBA)F1 melanoma
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Description
Description: Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.
Also Known As:
Species: Mouse
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Ham’s F10 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1966;154:1186
Hyperlink to ECACC Cell Line Data Sheet: 87081806
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