Description: COCA, a murine epidermal cell line was developed by serially passaging keratinocytes from the back skin of adult C57BL/DBA mice. Culture of the COCA cells is in fully defined media without requirement for feeder layer or other coating. COCA retains its ability to differentiate and stratify in response to increased calcium concentrations thus providing an excellent experimental system for in vitro (conventional and 3D epidermal cell cultures) and in vivo (skin regeneration) skin modelling.

Also Known As:

Species: Mouse

Tissue: Epidermis

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: CnT-07 media (CELLnTEC, Bern, Switzerland. http://www.cellntec.com/products/epidermal/culture-media/cnt-07) This defined medium contains low calcium (0.07mM). To promote good cell attachment post trypsinisation, an 8-14 hours incubation in CnT-07 medium containing 0.2mM CaCl2 is necessary. Differentiation can be initiated by transferring a confluent monolayer from 0.07mM calcium to high calcium (1.2mM). In Segrelles et al 2011 PMID: 21510892, 3D in vitro cultures were performed using CnT-02-3D cell culture media from CELLnTEC (Bern, Switzerland). The company has recently changed the formulation of this culture media to CnT-02-3D Prime (CnT-02-3DP) media. The ability of both media (CnT-02-3D Classic the original vs. Prime) to grow 3D cultures using COCA keratinocytes has been determined by the authors. They found that (see attachment below) the recently developed Cnt-02-3D Prime media is not efficient to sustain 3D culture of COCA cells. CELLnTEC have confirmed that they will produce and provide CnT-02-3D Classic when requested.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 0.5-1.5 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Release Conditions: No

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Originator:

References: Carmen Segrelles, Almudena Holguin, Pilar Hernandez, José M Ariza, Jesus M Paramio and Corina Lorz. 2011 Establishment of a murine epidermal cell line suitable for in vitro and in vivo skin modelling. BMC Dermatology Apr 21; 11(1):9 PMID: 21510892.

Hyperlink to ECACC Cell Line Data Sheet: 10112001