Description: Established from the pleural fluid of a patient who suffered with malignant fibrous histiocytoma. Colo 738 is also referred to as MFHL-2 in some literature. Cell line was mycoplasma eradicated by ECACC using (CIP). This cell line was found to be indistinguishable from cell lines RPMI 7932 & COLO 699N by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.

Also Known As:

Species: Human

Tissue: Unknown

Growth Properties: Adherent

Morphology:

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 using using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C; cells can show extensive blebs

Release Conditions: No

Depositor:

Originator: No

References: Malignant fibrous histiocytoma: A tumor of facultative histiocytes sharing mesenchymal differentiation in cultured cell lines. Cancer 1992 Jan 69:no.2 pp437-447.

Hyperlink to ECACC Cell Line Data Sheet: 94072238