CRE BAG2
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Description
Description: CRE BAG2 has been established from the NIH 3T3 fibroblast cell line. Cells were transfected with Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. CRE BAG2 produces BAG ( a ß-gal-transducing vector) and can be used for the package of vectors derived from murine leukaemia viruses. The production of recombinant replication-competent helper viruses has never been reported.
Also Known As:
Species: Mouse
Tissue: unknown
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1987;84:156; ibid 1988;85:6460
Hyperlink to ECACC Cell Line Data Sheet: 95082219
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