Description: The cells have been selected for sensitivity to SV40.

Also Known As:

Species: Monkey

Tissue: kidney

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 2% Tricine (1M solution at pH7.4) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union

Depositor: Prof F Horaud, Institut Pasteur, Paris

Originator: Yes

References: Proc Natl Acad Sci, USA 1964;53:53; Virology 1965;27:453

Hyperlink to ECACC Cell Line Data Sheet: 90032601