DAUDI
Human Burkitt's lymphoma
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Description
Description: Derived from a 16 year old male. Positive for EBNA, carries EBV markers, complement receptors, surface bound immunoglobulin and surface markers for the Fc fragment of IgG. The cells should be handled under laboratory containment level 2. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 2-9×100,000 cells/ml; 5% CO2; 37°C. Cell numbers will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals. Cell growth can be slow on recovery from frozen stock. Resuscitate using 20% FBS, seeding at 3-4 x 100, 000 cells/ml and keep culture flask standing up until exponential growth is established.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1968;28:1300; Lancet 1967;2:1068; Int J Cancer 1977;19:337
Hyperlink to ECACC Cell Line Data Sheet: 85011437
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