Description: Derived from pleural fluid of an adult female with primary carcinoma of the pharynx. The cells have the type B glucose-6-phosphate dehydrogenase.

Also Known As:

Species: Human

Tissue: Pharynx

Growth Properties: Adherent

Morphology: Heteroploid

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Proc Soc Exp Biol Med 1971;136:1187

Hyperlink to ECACC Cell Line Data Sheet: 87042205