DF
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Description
Description: The cell line DF has been derived by transfecting NIH-3T3 with human chromosome 7 DNA. It was originally established for the analysis of the cystic fibrosis locus and contains a transgenomic size of 3-4 Mb. Chromosomal fragments detected were 7q22-31.3 (met oncogene) and 7q22-7cen.
Also Known As:
Species: Human/mouse hybrid
Tissue: Unknown
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5%CO2; 37°C.
Release Conditions: No
Depositor: Dr P Scambler, Institute of Child Health, London, UK
Originator: Yes
References: Nucleic Acid Res 1986;14:7159
Hyperlink to ECACC Cell Line Data Sheet: 86072802
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