Description: This cell line has properties essential for study of metastasis of colon carcinomas; a reproducible pattern of metastatic behaviour and ability to be propagated both in vitro and in vivo to facilitate identification of biochemical correlates of metastasis.

Also Known As:

Species: Rat

Tissue: colon

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + F10 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or 2% Rat Serum.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Int J Cancer 1983;32:623; Int J Cancer 1987;39:248

Hyperlink to ECACC Cell Line Data Sheet: 90062901