Description: DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH)”, bombesin (BN), 17 beta-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.

Also Known As:

Species: Human

Tissue: Lung (liver metastasis)

Growth Properties: Semi-adherent aggregates

Morphology:

Growth Medium: Waymouth’s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Cells grow in large clumps.

Release Conditions: No

Depositor: Prof OS Pettengill and Dr G D Sorenson, Dartmouth Medical School, Lebanon, NH

Originator: Yes

References: Cancer 1980;45:906; Cancer 1981;47:1289; Regulatory Peptides 1982;4:59

Hyperlink to ECACC Cell Line Data Sheet: 95062827