E.H.IV (Elaine IV)
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Description
Description: Established by seeding peripheral blood lymphocytes from 20 year old female, 2 months after onset of infectious mononucleosis. EBV transformed B-lymphoblastoid line. The cells should be handled under laboratory containment level 2. Cells are particularly resistant to poliomyelitis and vesicular stomatis, viruses similar to other cell lines carrying EB virus.
Also Known As:
Species: Human
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 4-5×100,000 cells/ml; 5% CO2; 37°C. Subculture every 8 days by centrifugation. Maximun of 1-2 x 1,0,000 viable cells/ml is obtainable.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1968;59:94; J Am Med Assoc 1968;203:205
Hyperlink to ECACC Cell Line Data Sheet: 90121307
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