Ehrlich-Lettre Ascites strain E
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Description
Description: Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.
Also Known As:
Species: Mouse
Tissue: unknown
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Media change 3 times a week.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1965;34:725
Hyperlink to ECACC Cell Line Data Sheet: 87032503
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