EMT6/MTXR
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Description
Description: The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P (ECACC catalogue no. 96042344) by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.
Also Known As:
Species: Mouse
Tissue: Unknown
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1µg/ml methotrexate + 15% Foetal Bovine Serum (FBS). Resuscitate in drug-free medium; add drug once growth is fully established
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA;5% CO2; 37°C
Release Conditions: No
Depositor: Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX
Originator: Yes
References: J Natl Cancer Inst 1989; 81:1588
Hyperlink to ECACC Cell Line Data Sheet: 96042322
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