Description: The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (ECACC catalogue no. 96042344) by growth in verapamil (see reference).

Also Known As:

Species: Mouse

Tissue: Unknown

Growth Properties: Adherent

Morphology: Epithelial-like

Growth Medium: MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 µg/ml verpamil + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.

Release Conditions: No

Depositor: Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX

Originator: Yes

References: Twentyman, P.R. et al (1990) Br. J. Cancer, 61:279-84

Hyperlink to ECACC Cell Line Data Sheet: 96042319