Description: Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

Also Known As:

Species: Mouse

Tissue: Embryo blastocyst

Growth Properties: Adherent

Morphology: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells ECACC Catalogue 86032003 (irradiated or treated with mitomycin C to prevent cell division. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.

Growth Medium: DMEM + 2mM Glutamine + 1% NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Foetal Bovine Serum. Renewal of medium every 2-3 days is required.

Subculturing Procedure:

Release Conditions: No

Depositor:

Originator: No

References: Smith & Hooper (1987) Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9. PMID: 3569655.

Hyperlink to ECACC Cell Line Data Sheet: 08021401