Description: ESO26 was established from a primary tumour located in the gastroesophageal junction and distal oesophagus of a 56 year-old Caucasian male in 2000 involving lymph nodes and distant metastases. ESO26 was found to be tumourigenic in female NMRI nude mice or NOD-SCID mice.

 Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer.

Also Known As:

Species: Human

Tissue: Gastroesophageal junction and distal oesophagus

Growth Properties: Suspension

Morphology: Large spherical clumps of cells loosely adherent to the plastic

Growth Medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Maintain cultures between 2-9 x 100,000 cells/cm3. Cells are loosely adherent and can be dislodged by gentle tapping or use of 0.02% EDTA solution. 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr W.N.M. Dinjens, Department of Pathology, Josephine Nefkens Institute, Erasmus MC, University Medical Centre, Rotterdam PO Box 2040, 3000CA Rotterdam. Originator: Dr P Chaves Department of Pathology and Cytology, Portuguese Institute of Oncology, Lisbon, Portugal

Originator:

References: Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. 2010 Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. J Natl Cancer Inst. 102(4):271-4.PMID: 20075370.

 Whole-genome sequencing of nine esophageal adenocarcinoma cell lines

Hyperlink to ECACC Cell Line Data Sheet: 11012009