Description: Investigation of differentiation-inducible leukaemia cells is considered important in the understanding of leukaemic or neoplastic state. Several ascites lines derived from friend virus-induced erythoid leukemia show an erythroid morphology and also, unusually high haem synthesis. Most cultured lines of friend virus-induced leukemia in mice, are known to be inducible to differentiate towards erythocytes. F-5-5.F1 is susceptible to the induction by both dimethyl and butyric acid.

Also Known As:

Species: Mouse

Tissue: Spleen

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100µl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37°C; 5 – 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. To keep the cells in exponential growth, maintain cultures between 3-9 x100,000 cells/ml.

Release Conditions: No

Depositor:

Originator: No

References: Ikawa Y, Aide M, Inoue Y. Gann,1976,76, p767-70

Hyperlink to ECACC Cell Line Data Sheet: 99072803