Description: Established from normal embryonal tongue tissue of female cat (Felis catus). Cells support the replication of a wide range of feline viruses. Can undergo 50 population doublings.

Also Known As:

Species: Feline

Tissue: tongue

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2 37°C. Maintain at pH 7.1-7.3.

Release Conditions: No

Depositor:

Originator: No

References: None specified by depositor

Hyperlink to ECACC Cell Line Data Sheet: 90073002