Description: A subclone derived from Fao which is deficient in HGPRT (resistant to 5µg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen. GMO1.

Also Known As:

Species: Rat

Tissue: skin

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: Coon’s modified Ham’s F12 (or Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2×1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Media change three times a week.

Release Conditions: No

Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris

Originator: Yes

References: Cell Growth and Differentiation 1990; 1:519-526; Biochemie 1974; 56:1603-1611.

Hyperlink to ECACC Cell Line Data Sheet: 94101904