FM3Ats C1.T85
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Description
Description: A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (ECACC catalogue no. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.
Also Known As:
Species: Mouse
Tissue: breast
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 1-3×100, 000 cells/ml; 5% CO2; 33°C.
Release Conditions: No
Depositor:
Originator: No
References: Exp Cell Res 1980;126:407
Hyperlink to ECACC Cell Line Data Sheet: 87111904
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