FT
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Description
Description: Derived from the tongue of a normal female bull frog. Incubation is optimal at 25°C but growth at a slower rate can continue even at 4°C where cells may be stored for prolonged periods.
Also Known As:
Species: Frog
Tissue: tongue
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Distilled H2O + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10000 cells/cm² using 0.02% EDTA at 10°C; 25°C. Capable of growth down to 4°C. Flask should not be gassed. Optimum pH is 8.0
Release Conditions: No
Depositor:
Originator: No
References: Science 1964;144:1578
Hyperlink to ECACC Cell Line Data Sheet: 90102525
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