G-7
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Description
Description: Derived from a muscle tissue culture of a foetal Swiss-Webster mouse. Tumorigenic and only slightly sensitive to acetylcholine. When confluent cultures form short multinucleated myotubes.
Also Known As:
Species: Mouse
Tissue: muscle
Growth Properties: Adherent
Morphology: Myoblast
Growth Medium: DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin in Ca2+ + Mg2+-free PBS; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.
Release Conditions: No
Depositor:
Originator: No
References: Science 1977;196:995
Hyperlink to ECACC Cell Line Data Sheet: 90110520
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