GH1
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Description
Description: Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1µg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.
Also Known As:
Species: Rat
Tissue: pituitary
Growth Properties: Semi-adherent
Morphology: Epithelial
Growth Medium: Ham’s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells by centrifugation and reseed into fresh medium. Incubate overnight and the cells should begin to reattach.
Release Conditions: No
Depositor:
Originator: No
References: Science 1966;154:1186; Endocrinology 1968;82:342
Hyperlink to ECACC Cell Line Data Sheet: 89072610
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