GL-1
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Description
Description: From adult male Tokay Gekko (indigenous to Southeast Asia). Observed to undergo spontaneous chromosomal alterations from a normal diploid number of 38 to a bimodal distribution of chromosome number 38-40. Susceptible to a number of mammalian viruses at 36°C.
Also Known As:
Species: Lizard
Tissue: lung
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4. seeding at 4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 30°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Soc Exp Biol Med 1970;133:1039; Cytogenetics 1968;7:16
Hyperlink to ECACC Cell Line Data Sheet: 89072612
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