GM1899A
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Description
Description: The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).
Also Known As:
Species: Human
Tissue: Unknown
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates
Release Conditions: No
Depositor:
Originator: No
References: Free Radic Biol Med 1997;22:717
Hyperlink to ECACC Cell Line Data Sheet: 98120701
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