Description: GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

Also Known As:

Species: Rat

Tissue: Brain

Growth Properties: Adherent

Morphology: Fusiform

Growth Medium: Hams F10 + 10% Heat-inactivated FCS + 2mM Glutamine + 2ng/ml basic fibroblast growth factor + 80µg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10µg/cm² from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to ammonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 10hrs.

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor: Professor John Greenwood, University College London Institute of Opthalmology, University College London, Bath Street London ECIV 9EL.

Originator: Yes

References: Greenwood et al. (1996) SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology 71: 51-63. PMID: 8982103.

Hyperlink to ECACC Cell Line Data Sheet: 09061601