Description: From a primary hepatocyte culture of 6 week old female BALB/c mouse transformed with tsA255 (temperature-sensitive mutant of SV40 virus). H2.35 is temperature sensitive for expression of serum albumin mRNA. During routine propagation at 33°C it exhibits extremely low levels of albumin transcription and mRNA, while at the restructure temp of 39°C albumin mRNA increases 100 fold if the cells are grown in serum free medium on a collagen substratum. Undergoes morphological changes in response to extracellular matrix gel substrata, albumin gene transcription is induced. Liver-enriched transcription factors are induced in serum-free medium and on collagen gels. The cells should be handled under laboratory containment level 2.

Also Known As:

Species: Mouse

Tissue: liver

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM (containing 1g / l glucose) + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 4% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33°C.

Release Conditions: No

Depositor:

Originator: No

References: Proc Natl Acad Sci, USA 1988;85:9076; Mol.Cell.Biol 1990;10:4582, Mol.Cell.Biol 1990;11:773, Mol.Cell.Biol. 1991;11:4405

Hyperlink to ECACC Cell Line Data Sheet: 94050407