H4S
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Description
Description: Has been infected with a temperature sensitive mutant of VSV which is defective in RNA replication and transcription.
Also Known As:
Species: Rat
Tissue: liver
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cell 1980;2:709
Hyperlink to ECACC Cell Line Data Sheet: 89102001
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