H9c2 (2-1)
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Description
Description: Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.
Also Known As:
Species: Rat
Tissue: heart
Growth Properties: Adherent
Morphology: Myoblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.
Release Conditions: No
Depositor:
Originator: No
References: Exp Cell Res 1976;98:367, J. Biol. Chem 1996;271:2746-2753
Hyperlink to ECACC Cell Line Data Sheet: 88092904
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